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MRSA in the NICU

Outbreak or Coincidence?



Co Authors:

Maria P. Chadiarakou
Bioinformatics
National Center for Genome Resources
mhadres@ncgr.org

Anitha Sundararajan
Bioinformatics
National Center for Genome Resources
asundara@ncgr.org

Ingrid E. Lindquist
Health Sciences Center
University of New Mexico
ingrid.e.lindquist@gmail.com

Gabriella A. DeFrancesca

Desert Academy

Madeline Kwicklis

The Masters Program

Drew A. Lighthall

Tierra Encantada

Natasha E. Farmer


Michèle I. Shuster
Department of Biology
New Mexico State University
mshuster@nmsu.edu

Joann Mudge
Bioinformatics
National Center for Genome Resources
jm@ncgr.org

Abstract:

DNA sequencing and analysis (bioinformatics) is an increasingly important tool for understanding biological data, including medical data. In this case study, students will be exposed to DNA sequencing to quickly identify how an outbreak of methicillin-resistant Staphylococcus aureus spreads through a neonatal intensive care unit (NICU). This PowerPoint-driven case is based on an actual outbreak described in the New England Journal of Medicine and students will use the actual DNA sequences from the outbreak that were used by scientists. A handout (Supplemental Materials) provides background and instructions that guide students in how to create and read a phylogenetic tree and use to it identify which organisms are part of the outbreak and which are independent MRSA infections. They will then use this data to assess the containment strategies used at the hospital during the outbreak. Students will learn how DNA sequencing can inform epidemiology to identify outbreak cases, track trajectories of the spreading disease, and quickly put containment measures into place. This case study is especially suited for high school and introductory undergraduate biology classes.

Objectives:
  • Generate two phylogenetic trees, one based on antibiotic resistance (phenotype), and the other based on DNA sequences from patient infections.
  • Interpret the two phylogenetic trees to analyze an outbreak in a hospital environment.
  • Analyze the strengths and weaknesses of phenotype (antibiotic resistance profile) vs. genotype-based (DNA sequencing) phylogenetic trees for analyzing a hospital-based outbreak.
  • Develop a fuller understanding of evolution by natural selection, phylogenetic trees, bioinformatics tools, and medical applications of bioinformatics tools and information.
Keywords: NICU; outbreak; MRSA; antibiotic resistance; phylogeny; DNA sequencing; bioinformatics
Topical Area: N/A
Educational Level: High school, Undergraduate lower division, Undergraduate upper division, Clinical education, General public & informal education
Formats: PDF, PowerPoint
Type/Method: Analysis (Issues), Directed, Discussion, Journal Article
Language: English
Subject Headings: Biology (General)   Epidemiology   Genetics / Heredity   Medicine (General)   Evolutionary Biology   Public Health   Science (General)  
Date Posted: 8/29/2018
Date Modified: N/A
Copyright: Copyright held by the National Center for Case Study Teaching in Science, University at Buffalo, State University of New York. Please see our usage guidelines, which outline our policy concerning permissible reproduction of this work.

Teaching Notes


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Answer Key


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Supplemental Materials


The following handout and datasets are used in this case study.

  Case Study Handout
  Genetic (sequencing) data in phylip interleaved format
  Phenotypic (antibiotic resistance) data in binary format (sensitive or resistant)



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