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In Search of a Cure for Breast Cancer



Author:

Jolanta Skalska


jskalska@gmail.com

Abstract:

In this directed case study, students analyze data, draw a research-based conclusion, interpret experimental results, and discuss the relevance of research findings for clinical practice. Specifically, students examine the effects of chemotherapeutic drugs on newly generated cell lines and explain research outcomes using their prior knowledge of signal transduction pathways (G-protein coupled receptors), hormones, glycolysis, oxidative phosphorylation, and DNA structure and function as they follow the story of "Emily," an undergraduate student who is accepted into an internship program focusing on the breast cancer cell line MCF-7. Emily learns that MCF-7 cells can survive the treatment of tamoxifen and a hormone deprivation regimen, which leads to the generation of new cell lines (Tam3 and TamR3) that do not activate the mTOR signaling pathway. Emily attempts to predict how the Tam3 and TamR3 cells will respond to the mTOR inhibitor rapamycin, and then incorporates drugs used for chemotherapy into her experiments. Originally written for upper-level undergraduate biology majors, the case study is also appropriate for courses focusing on cell biology, pharmacology, and cancer biology.

Objectives:
  • List the functions of the steroid hormones, classify receptors, and summarize the mechanism of action and effects of chemotherapeutic drugs.
  • Interpret experimental data and explain the outcomes of experiments described in the case study.
  • Determine the differences between cell lines based on data, present signaling transduction pathways, and predict research outcomes.
  • Explain the phenotypical differences between three cell lines, debate experimental outcomes and present them in the form of a written discussion.
Keywords: Breast cancer; camptothecin; competitive inhibitor; cisplatin; doxorubicin; fluorouracil; G-protein coupled receptors; MCF-7; ER+; PR+; membrane receptors; mTOR; signaling pathway; oxidative phosphorylation; reactive oxygen species; tamoxifen
Topical Area: N/A
Educational Level: Undergraduate upper division
Formats: PDF
Type/Method: Directed, Discussion
Language: English
Subject Headings: Biochemistry   Cell Biology   Molecular Biology   Pharmacy / Pharmacology  
Date Posted: 10/23/2019
Date Modified: N/A
Copyright: Copyright held by the National Center for Case Study Teaching in Science, University at Buffalo, State University of New York. Please see our usage guidelines, which outline our policy concerning permissible reproduction of this work.

Teaching Notes


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Answer Key


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Supplemental Materials


The optional PowerPoint presentation below can be used to pace students as they work through the case study in class.

  breast_cancer_direct.pptx (~100 KB)



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