New search
download case
  • Overview
  • Teaching Notes
  • Answer Key
  • Comments/Replies

Resurrection

Reviving a Deadly Flu Virus



Author:

Karobi Moitra
Department of Biology
Trinity Washington University
MoitraK@trinitydc.edu

Abstract:

This case study examines the molecular methods that were used to reverse engineer the 1918 influenza virus strain in order to try and solve the mystery of why it was so deadly. The story starts in the 1950s with the unsuccessful attempts to culture the influenza virus and follows scientists through to the turn of the century when cutting edge molecular tools enabled scientists to finally resurrect the 1918 virus through reverse genetics. The history and methods involved in resurrecting this deadly virus are reviewed in class with a PowerPoint presentation containing clicker questions (answered with a personal response system) and discussion questions (answered in small groups). This "clicker case" is suitable for high school biology and lower division undergraduate biology classes for non-majors. It could also be used in any lower division non-major class focused on human disease and the history of human disease.

Objectives:
  • Explain the basic components of molecular cloning.
  • Explain the process of reverse genetics.
  • Describe the process of PCR and sequencing.
  • Explain the concepts of reverse engineering.
  • Decipher the history behind the science.
  • Describe the reverse engineering of the 1918 influenza virus.
  • Describe the 1918 influenza pandemic and its consequences.
Keywords: influenza virus; reverse genetics; 1918 pandemic; Spanish flu; molecular cloning; PCR; Hultin; Taubenberger; hemagglutinin
Topical Area: History of science
Educational Level: High school, Undergraduate lower division, General public & informal education
Formats: PDF, PowerPoint
Type/Method: Clicker
Language: English
Subject Headings: Biology (General)   Biomedical Engineering   Microbiology   Molecular Biology   Public Health  
Date Posted: 8/8/2016
Date Modified: N/A
Copyright: Copyright held by the National Center for Case Study Teaching in Science, University at Buffalo, State University of New York. Please see our usage guidelines, which outline our policy concerning permissible reproduction of this work.

Teaching Notes


Case teaching notes are password-protected and access to them is limited to paid subscribed instructors. To become a paid subscriber, begin the process by registering here.

Teaching notes are intended to help teachers select and adopt a case. They typically include a summary of the case, teaching objectives, information about the intended audience, details about how the case may be taught, and a list of references and resources.

  Download Teaching Notes

Answer Key


Answer keys for the cases in our collection are password-protected and access to them is limited to paid subscribed instructors. To become a paid subscriber, begin the process by registering here.


  Get Answer Key



Name:
Email:
Department:
Institution:
City State:
Comments:
security code
Enter Security Code: