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Rice ’n Beans or Ricin Beans?

A Deadly Swap


Author(s)

Ann T.S. Taylor
Department of Chemistry
Wabash College
taylora@wabash.edu

Abstract

Taylor must write a report about a natural toxin while she is home from college on break. After a family dinner conversation about the latest attempt to poison a politician via a letter, Taylor decides to explore how ricin acts as a poison. Students work in small groups to help Taylor by working through figures from primary literature papers and exploring the use of an in vitro translation system, sucrose gradient fractionation and the effect of inhibiting various steps of translation.  A shorter, second day activity involves students looking further at the effect of ricin upon ribosome function and at the ricin protein itself.  Students individually complete a cumulative assignment of writing a letter back to Mom and Dad about how ricin has its effects. This case was designed for use in a second semester biochemistry course or a molecular biology course that incorporates the mechanism of transcription. Prerequisite knowledge includes a general understanding of the steps of translation and the ability to interpret data from agarose gels.


Objectives

  • Describe the steps of translation in molecular detail.
  • Interpret experimental evidence that can be used to monitor translation, including radionucleotide tracers, polyribosome isolation, and modification.
  • Apply and extend the concepts of translation to predict and explain inhibitor effects.
  • Form hypotheses and logical experimental techniques to explore translation.

Keywords

ricin; translation; experimental design; primary literature; in vitro translation; ribosome; toxin; poison

Topical Areas

N/A

Educational Level

Undergraduate upper division

Format

PDF, PowerPoint

Type / Methods

Jig-Saw, Journal Article

Language

English

Subject Headings

Biochemistry  |   Biology (General)  |   Genetics / Heredity  |   Molecular Biology  |  


Date Posted

7/1/2016

Teaching Notes

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Supplemental Materials

The PowerPoint presentation below contains the figures that appear in the case study.

  
  ricin.pptx

Answer Key

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